ABO and RhD Blood Grouping and Typing
Q: Which automated technology for ABO grouping uses a microcolumn containing gel?
Did You Know?
The Fetomaternal Hemorrhage (FMH) test is performed to **detect and quantify the transfer of fetal red blood cells into the maternal circulation**. The most common test is the **Kleihauer-Betke (KB) acid elution test**, though flow cytometry is now often used for greater precision. **When is it performed?** 1) **Routine Postpartum for Rh-Negative Mothers**: To determine if a significant FMH (>15 mL) has occurred that would require additional doses of Rh immune globulin (RhIg) beyond the standard 300 µg dose. 2) **After Potentially Sensitizing Events During Pregnancy**: Such as trauma, abdominal surgery, amniocentesis, chorionic villus sampling, external cephalic version, or vaginal bleeding. 3) **Unexplained Positive Direct Antiglobulin Test (DAT) in a Newborn**: To assess the possibility of large FMH causing HDFN. 4) **Evaluation of Antepartum Bleeding**. The test quantifies the percentage of fetal cells (HbF-positive) in a maternal blood sample, allowing calculation of the volume of fetal blood that has entered the mother's circulation. This volume dictates the number of RhIg vials needed to prevent Rh immunization.
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