True. Polymer-based detection systems, such as the widely used EnVision systems, utilize a synthetic polymer backbone, often composed of dextran (a large sugar molecule). To this backbone, a high number of enzyme molecules (like horseradish peroxidase or alkaline phosphatase) and numerous secondary antibody molecules are chemically conjugated. This creates a large, stable complex. During staining, after the primary antibody binds, this single polymer complex reagent is applied. The secondary antibody components on the polymer bind to the primary antibody, delivering dozens of enzyme molecules directly to the antigen site in one step. This architecture provides tremendous signal amplification, high sensitivity, and simplicity (often a two-step protocol). It also avoids the issues associated with the biotin-avidin interaction, such as background from endogenous biotin. Polymer systems are a cornerstone of modern automated IHC.

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