Calculating Order Quantities and the eLMIS
Q: What is the formula to calculate the maximum stock quantity (Max) in the ordering formula OQ = Max – SOH?
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In the context of IHC, the 'limit of detection' (LOD) is the lowest amount or concentration of the target antigen that the assay can reliably distinguish from background (a negative result). It is a measure of the assay's sensitivity. For a semi-quantitative method like IHC, the LOD is often determined experimentally by testing the antibody on a series of control tissues or cell lines with known, decreasing levels of antigen expression. The LOD is the point at which the staining signal becomes consistently visible and reproducible above the background noise observed in a true negative sample. Establishing the LOD is crucial for assay validation, especially for biomarkers where low-level expression is clinically relevant. It helps ensure the assay is fit for its intended purpose and guides the interpretation of weakly stained samples.
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