Cell Signalling Pathways in Disease (MAPK & PI3K/Akt)
Q: What is the role of the protein 4E-BP1 in the PI3K/mTORC1 pathway?
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Optimal storage conditions for urine cytology specimens depend on timing: 1) For immediate processing (<2 hours), room temperature is acceptable. 2) For delayed processing (2-24 hours), refrigeration at 2-8°C is recommended to slow cellular degeneration. 3) For longer delays (>24 hours) or transport, preservation with an equal volume of 50-70% ethanol or commercial cytology fixative (e.g., Cytolyt) is necessary. Boric acid can be added as a preservative if microbiology testing is also needed. Unpreserved urine should never be frozen as ice crystals rupture cells. Proper labeling and leak-proof containers are essential for all storage conditions.
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