True. The EnVision system (and similar systems with other brand names) is a widely used, commercially available two-step polymer-based detection method. It revolutionized IHC by simplifying protocols and increasing sensitivity while reducing background. Here's how it works: In Step 1, the primary antibody binds to the antigen. In Step 2, a ready-to-use reagent is applied. This reagent consists of a dextran polymer backbone to which a large number of secondary antibody molecules and horseradish peroxidase (HRP) enzyme molecules are conjugated. This single reagent simultaneously acts as the secondary antibody and the enzyme label. The secondary antibodies on the polymer bind to the primary antibody, delivering dozens of HRP enzymes directly to the antigen site. This provides massive signal amplification in one step after the primary antibody. The method is fast, sensitive, and avoids the biotin-avidin interaction, eliminating problems with endogenous biotin. Its simplicity and reliability have made it a workhorse in both manual and automated IHC.

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