Flow Cytometry Principles and Applications
Q: Flow cytometry can only measure the physical characteristics of cells, not their fluorescence.
Did You Know?
True. Each PCR cycle includes a high-temperature step (around 95°C) to denature (separate) the DNA double helix. A regular DNA polymerase would be destroyed at this temperature. Taq polymerase, isolated from a thermophilic bacterium, remains stable and active throughout these cycles, making automated PCR possible.
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