A fluorescence microscope is absolutely required to visualize the results of a standard immunofluorescence (IF) assay. Fluorescence microscopes are equipped with specific light sources (typically mercury or LED lamps or lasers) that produce high-intensity light at wavelengths needed to excite the fluorochromes attached to the antibodies. They also have specialized filter sets: an excitation filter to select the correct wavelength of light to illuminate the specimen, a dichroic mirror to direct this light onto the sample, and an emission filter to block the exciting light and allow only the longer-wavelength emitted fluorescence from the specimen to pass through to the eyepieces or camera. This allows the faint fluorescent signal to be seen against a dark background. A standard brightfield microscope, used for viewing H&E or enzymatic IHC stains, cannot visualize fluorescence because it uses transmitted white light and lacks the necessary filters and often the powerful light source.

Break down complex topics into smaller, manageable chunks for easier recall.

Zambia

• Joseph Soko – 101 pts

• Visual Pathway Lesions
• Positive and Negative Symptoms
• Hallucinations and Delusions
• Antipsychotic Medications
• Emergency: Environmental Emergencies