True. Immunoelectron microscopy (Immuno-EM) is a powerful technique that merges the principles of immunohistochemistry with the high resolution of electron microscopy. It allows for the precise subcellular localization of antigens at the nanometer scale, far beyond the resolution limit of light microscopy. In this method, antibodies specific to the target antigen are labeled with an electron-dense marker. The most common markers are colloidal gold particles of defined sizes (e.g., 5nm, 10nm), which appear as discrete black dots under the electron beam. The tissue is processed for EM (often lightly fixed and embedded in a resin that preserves ultrastructure), and the labeled antibodies are applied. The gold particles precisely mark the location of the antigen, revealing if it is on the plasma membrane, within specific organelles like mitochondria or the Golgi apparatus, or associated with cytoskeletal elements. This technique is invaluable in cell biology and pathology for understanding the detailed function and trafficking of proteins within cells.

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