Presumptive and Confirmatory Testing in Forensics
Q: True or False: In forensic science, presumptive tests provide definitive identification of a substance.
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This test distinguishes fetal red cells from maternal red cells based on their hemoglobin content. Fetal red cells contain fetal hemoglobin (HbF), which is resistant to acid elution. In the Kleihauer-Betke test, a maternal blood smear is treated with acid, which elutes adult hemoglobin (HbA) from maternal cells, leaving them as 'ghosts.' Fetal cells, with their HbF intact, stain pink. This quantifies the volume of fetomaternal hemorrhage (FMH), which is critical for determining the correct dose of Rh Immunoglobulin.
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