University revision · Parasitology

Trypanosoma Cruzi and Chagas Disease

Question 1 of 11 Course: Biomedical Science(Degree)

Q1 Essay · up to 5 points

What is the term for the enlarged, fluid-filled colon often seen in the chronic phase of Chagas disease?

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Omission of the counterstain is NOT a typical cause of high background staining. High background refers to non-specific, diffuse coloration across the tissue that obscures the specific signal. The counterstain (e.g., hematoxylin) is applied after the specific IHC reaction to provide contrast by staining nuclei; it does not interact with the IHC detection chemistry. Its omission would result in a slide with only the specific chromogen stain (e.g., brown DAB) and a clear, colorless background—this might make evaluation difficult due to lack of tissue context, but it does not create a high, diffuse, non-specific version of the chromogen's color. Typical causes of high background include: insufficient blocking of non-specific sites, endogenous enzyme activity not quenched, too high concentration of primary or secondary antibody, over-development of the chromogen, dried sections during incubation, and non-specific binding of detection system components (e.g., due to ionic interactions with avidin).

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