Direct Antiglobulin Test (Coombs Test)

The Direct Antiglobulin Test (DAT), also known as the Coombs test, is a serological assay used to detect antibodies or complement proteins attached to the surface of red blood cells. This topic is essential for tertiary students in Zambia studying hematology, immunology, or medical laboratory science. It covers the principle, procedure, clinical indications, and interpretation of DAT in diagnosing autoimmune hemolytic anemia, hemolytic disease of the newborn, and drug-induced immune hemolysis. Understanding DAT helps learners differentiate between immune and non-immune hemolytic disorders, supporting accurate diagnosis and patient management. Mastery of this test is crucial for examination preparation and professional practice in Zambian hospitals and diagnostic laboratories. The topic aligns with international hematology standards and prepares students for certification and career advancement in clinical pathology and transfusion medicine.