Cell blocks from sputum specimens are prepared by: 1) Concentrating the specimen through centrifugation after mucolysis, 2) Forming a cell pellet, 3) Fixing the pellet in 10% neutral buffered formalin for at least 4 hours, 4) Processing through graded alcohols and xylene, 5) Embedding in paraffin, and 6) Sectioning at 4-5 μm thickness. Alternative methods include using plasma-thrombin to form a clot or commercial cell block systems (e.g., Cellient). Cell blocks allow for multiple sections for special stains (acid-fast, fungal), immunohistochemistry, and molecular testing. They are particularly useful when diagnostic material is limited or when architectural assessment is needed.

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Zambia

• loveness Phiri – 1084 pts

• Visual Pathway Lesions
• Positive and Negative Symptoms
• Hallucinations and Delusions
• Antipsychotic Medications
• Emergency: Environmental Emergencies