Nutrition in Disease and Stress Conditions
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The differentiation between A1 and A2 red blood cells is commonly performed using a lectin extracted from the seeds of the Dolichos biflorus plant. This lectin, called Dolichos biflorus lectin, has a specific affinity for the A1 antigen. When mixed with red cells, it will cause strong agglutination of A1 cells but will cause very weak or no agglutination of A2 cells. This provides a simple and reliable serological method to classify A subgroups. The test is performed by mixing a drop of the patient's washed red cells with a drop of the diluted lectin reagent, incubating briefly at room temperature, and observing for agglutination. Strong agglutination confirms the A1 subgroup, while weak or negative agglutination suggests A2 or another weaker A subgroup.
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