Viral Epidemiology & Public Health
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False. The SOP explicitly states NOT to incubate culture plates in CO2 except for specific fastidious organisms like Haemophilus spp., N. gonorrhoeae, and Streptococcus pneumoniae. For most non-fastidious bacteria, incubation in ambient air is standard, as CO2 can alter the pH of the medium and affect antibiotic activity and zone sizes.
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