Microtomy

Microtomy is a fundamental laboratory technique in tertiary-level cellular pathology and histotechnology courses, widely taught in Zambian universities and colleges offering biomedical sciences, laboratory technology, and medical degrees. This topic covers the science and practice of cutting thin tissue sections for microscopic examination, an essential skill for diagnostic pathology, research, and clinical laboratory practice. Students will learn about various types of microtomes—including rotary, rocking, sliding, sledge, freezing, and ultra-microtomes—each suited for different tissue types and analytical requirements. The module also includes detailed instruction on microtome components, knife types, sectioning procedures, flotation baths, slide preparation, and troubleshooting common cutting faults. Mastery of microtomy is critical for producing high-quality histological slides, accurate diagnosis, and valid research outcomes. For Zambian tertiary learners pursuing diplomas or degrees in medical laboratory science, pathology, or biotechnology, proficiency in microtomy supports success in examinations, practical assessments, and readiness for careers in hospitals, research institutions, and public health laboratories across Zambia.