Single-Cell RNA Sequencing (scRNA-seq)

Single-Cell RNA Sequencing (scRNA-seq) is an advanced genomic technique that enables transcriptome analysis at the individual cell level, providing unprecedented insights into cellular heterogeneity, development, and disease mechanisms. For tertiary students in Zambia, particularly in biomedical sciences and biotechnology programs, this topic covers the fundamental differences between scRNA-seq and bulk RNA-seq, the detailed workflow from single-cell isolation and lysis to library preparation, sequencing, and high-dimensional data analysis. Learners will explore major real-world applications in cancer research, immune system studies, neurological disorders, and drug discovery, understanding how scRNA-seq identifies rare cell populations, characterizes tumor microenvironments, and maps cellular trajectories. The topic also addresses the integration of scRNA-seq with other omics data (genomics, proteomics) and the role of AI/ML in data analysis, preparing students for the complexities of modern multi-omics research. Mastering scRNA-seq equips Zambian university and college graduates with skills crucial for careers in academic research, clinical diagnostics, and biotechnology innovation, supporting national goals in precision medicine and advanced healthcare solutions. Examinations test the ability to design scRNA-seq experiments, interpret cellular heterogeneity data, and evaluate integration strategies, ensuring competency in this transformative technology.