RNA Sequencing (RNA-seq)

RNA Sequencing (RNA-seq) is a next-generation sequencing (NGS) technology essential for Zambian tertiary students in advanced biotechnology and molecular biology. It enables comprehensive analysis of RNA quantity and sequences within biological samples. Students learn the workflow from mRNA extraction and cDNA library preparation to sequencing on NGS platforms and bioinformatic data analysis. This topic covers bulk RNA-seq and single-cell RNA-seq (scRNA-seq), along with applications in differential gene expression, transcriptome analysis, fusion gene detection, non-coding RNA study, and alternative splicing. RNA-seq offers high sensitivity, a wide dynamic range, and the ability to detect novel transcripts. For Zambian university and college learners, proficiency in RNA-seq supports careers in genomics, biomedical research, and molecular diagnostics, aligning with global advances in precision medicine.